I’ve seen them automatically inserted by the host, the ‘.well-known’ folder and the associated htaccess rules are something to do with site secutity. I found this online about it:
The “ .well-known” directory will appear on all cPanel servers that are using the Comodo AutoSSL feature. The directory will contain one or more .txt files that Comodo will use to validate the domain name before it will issue an SSL Certificate for it.
If what you want to do is simply remove the trailing / from Any URL that ends with one this RedirectMatch should work. I don’t know if this will solve the problem you are having since I’ve not heard of anyone needing to do that.
Many thanks everyone for your responses. Doug, it actually turned out that the folder got loaded into the cPanel, not the file, even though it showed the file sitting there. Weird. Anyway, thank you so much for the time spent on this. Much appreciated.